LUTON Town sent FA Trophy holders Wrexham crashing out of the competition with a 2-0 victory at Kenilworth Road on Saturday.
The scoreline might not indicate a one-sided affair, but Grimsby Town's Conference Premier rivals could have put the match beyond the Welsh side by half-time.
A Mark Cullen double, either side of the break, proved to be enough for the Hatters against struggling Wrexham, who beat the Mariners in the 2012/2013 showpiece.
Meanwhile, Eastleigh pulled off a real shock by beating Gateshead 2-0 to reach the last 16.
The Conference South side were playing against an outfit from the league above, but that gap did not show as the took the game to the Head from the outset and deservedly claimed the victory.
A crowd of 330 braved the windy conditions in Hampshire to watch the sides, and they were rewarded by a fine performance from the hosts.
And after 32 minutes Jamie Collins gave them a deserved lead, smashing home from outside the area.
Gateshead pushed forward early in the second half, but were undone by another quality strike with 18 minutes left.
This time it was Stuart Fleetwood who grabbed the goal, lashing home a half-volley from 20 yards.
North Ferriby United produced the performance of the second round as they beat Woking 4-0.
Two goals from Liam King and one each from Anthony Wilson and Danny Clarke did the damage for the Conference North League side against their Conference National opponents.
The game was drifting towards a half-time stalemate when Wilson blasted home from six yards, while King and Wilson did the second half damage.
Elsewhere, there was an impressive away win for Hungerford Town, who won 1-0 at Leek Town.
Cambridge United went to St Albans and booked their place in the Third Round with another 2-1 victory, as did Ebbsfleet United at Whitstable Town.
Lincoln City won 3-1 at Braintree Town, Maidenhead United won 2-0 at Barrow and Guiseley scored three unanswered goals against neighbours Bradford PA.
Tamworth beat Boston United 2-0.